Flexible DNA isolation procedure for different tree species as a convenient lab routine

Abstract DNA isolation is a fundamental technique for all molecular biology laboratories. Depending on the plant species, can be challenging. In particular, adapted protocols rarely exist tree species which are not used as standard model organisms. Here, we describe flexible protocol that works 59 in modular system. It based an ATMAB-containing extraction buffer to proteinase K and/or boric acid added, depending species. Subsequent purification steps include one or two precipitations with dichloromethane and, optional sodium acetate precipitation. Using leaf material of hybrid poplar clone from vitro culture, it was determined higher amounts could isolated this than field leaves. Starting material, difficult cases achieved cambium root tissue. This extract subsequent PCR amplification. Markers cpDNA, mtDNA, and genomic were standardized testing.